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cyklodextrin — Engelska översättning - TechDico

The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced. The purified Y93F CGTase was 76.39 kDa based on 10% SDS-PAGE analysis and showed both β-cyclization and starch hydrolysis activities in a zymography assay.

Cgtase

Due to their potential properties, CDs had been discovered to more. The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded The CGTase enzyme obtained from the M5 clone had Km and Vmax values 13.59 mg/mL and 1153 µmol/(mg min), respectively.

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Väger 145 g. · imusic.se. Den framställs genom att glukosyltransferas (CGTase, EC 2.4.1.19) får reagera med hydrolyserad stärkelse.

rekombinant - English translation – Linguee

B. circulans, B. macerans and B. stearothermophilus) and Brevibacillus brevis. [1] This enzyme belongs to the family of glycosyltransferases , specifically the hexosyltransferases. CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - CGTase is an important industrial enzyme that can transfer starch to glycosyl groups to form cyclodextrin (Li et al., 2014c; Qi et al., 2007). CGTase has been found in many bacteria, including Bacillus spp.

Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation. GO Commonly cyclodextrin glycosyltransferase (CGTase) is employed along with α-amylase. First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. Immobilisation of CGTase for continuous production of long-carbohydrate-chain alkyl glycosides Control of product distribution by flow rate adjustment.
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cgtase bacteria cyclodextrin gene starch Prior art date 1990-03-27 Application number PCT/EP1991/000560 Other languages German (de) French (fr) Other versions WO1991014770A3 (en Inventor Gerhard Schmid Original Assignee Consortium für elektrochemische Industrie GmbH Priority date (The priority date is an assumption and is not a legal conclusion. T1 - Substrate complexation and aggregation influence the cyclodextrin glycosyltransferase (CGTase) catalyzed synthesis of alkyl glycosides. AU - Zehentgruber, Daniela. AU - Lundemo, Pontus. AU - Svensson, David.

Status. Unreviewed-Annotation score: -Protein inferred from homology i.
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PY - 2011. Y1 - 2011 cgtase.

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The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis. The novel CGTase (CspCGT13) was also subjected to multiple sequence alignment with other well-characterized CGTases. From the alignment, the CGTase-specific sequence conservation was found in the four conserved regions identified for amylolytic enzymes (regions I, II, III and IV) (Table II) (MacGregor et al. 2001). 2008-08-26 · The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp.